1PZ8

Modulation of agrin function by alternative splicing and Ca2+ binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	76.847, 56.556, 84.586
Unit cell angles	90.00, 99.75, 90.00

Refinement procedure

Resolution	20.000 - 2.350
R-factor	0.21991
Rwork	0.218
R-free	0.26023
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.535
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.800
High resolution limit [Å]	2.350
Number of reflections	29221
<I/σ(I)>	11.3
Completeness [%]	97.1
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	276	PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K