1PXW

Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	67.190, 112.550, 34.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.940
R-factor	0.229
Rwork	0.227
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.233
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.030
High resolution limit [Å]	2.900 *	2.900 *
Rmerge	0.084 *	0.231 *
Total number of observations	18984 *
Number of reflections	5958 *
Completeness [%]	91.0 *	77.5 *
Redundancy	3.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	293	PEG MME 2000, magnesium chloride, sodium acetate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		300 (mM)
3	1	drop	Tris-HCl	20 (mM)	pH8.0
4	1	reservoir	PEG2000 MME	20 (%(v/v))
5	1	reservoir		160 (mM)
6	1	reservoir	sodium acetate	100 (mM)	pH4.0