1PVX

DO-1,4-BETA-XYLANASE, ROOM TEMPERATURE, PH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-02-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	38.762, 54.058, 90.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.590
R-factor	0.191
Rwork	0.191
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yna
RMSD bond length	0.015
RMSD bond angle	1.300
Data reduction software	AUTOMAR
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.590	1.590
Rmerge	0.093	0.358
Number of reflections	24200
<I/σ(I)>	25.71	3.19
Completeness [%]	92.5	78
Redundancy	5.13	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.50