1PS3
Golgi alpha-mannosidase II in complex with kifunensine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9504 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.089, 109.803, 138.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.200 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 * | 0.180 |
| Total number of observations | 759620 * | |
| Number of reflections | 144642 | |
| <I/σ(I)> | 17.2 | 4.1 |
| Completeness [%] | 94.2 * | 83.3 |
| Redundancy | 5.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | PEG 6000, MPD, Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 100 (mM) | pH7 |
| 2 | 1 | reservoir | PEG6000 | 8.5 (%) | |
| 3 | 1 | reservoir | MPD | 2.5 (%) |
