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1PRG

LIGAND BINDING DOMAIN OF THE HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]103
Detector technologyIMAGE PLATE
Collection date1997-06-01
DetectorRIGAKU
Spacegroup nameC 1 2 1
Unit cell lengths91.870, 62.000, 118.340
Unit cell angles90.00, 102.66, 90.00
Refinement procedure
Resolution60.000 - 2.200
R-factor0.246
Rwork0.246
R-free0.31800
Structure solution methodMIR
RMSD bond length0.006
RMSD bond angle18.000

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASES
Refinement softwareCNS (0. 0.3)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]60.0002.300
High resolution limit [Å]2.2002.200
Rmerge0.0520.052
Total number of observations89611

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Number of reflections27817

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<I/σ(I)>136.3
Completeness [%]85.068
Redundancy4.11.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

8.5

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VAPOR DIFFUSION METHOD, HANGING DROP TECHNIQUE, 1.4M SODIUM CITRATE, 100MM HEPES, PH 7.5
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein15 (mg/ml)
21dropTris-HCl20 (mM)
31dropdithiothreitol5 (mM)
41drop100 (mM)
51dropEDTA2 (mM)
61dropsodium citrate1.4 (M)
71dropHEPES100 (mM)
81reservoirsodium citrate1.4 (M)
91reservoirHEPES100 (mM)

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