1PRG
LIGAND BINDING DOMAIN OF THE HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.870, 62.000, 118.340 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.200 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.31800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (0. 0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 | 0.052 |
| Total number of observations | 89611 * | |
| Number of reflections | 27817 * | |
| <I/σ(I)> | 13 | 6.3 |
| Completeness [%] | 85.0 | 68 |
| Redundancy | 4.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | VAPOR DIFFUSION METHOD, HANGING DROP TECHNIQUE, 1.4M SODIUM CITRATE, 100MM HEPES, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | EDTA | 2 (mM) | |
| 6 | 1 | drop | sodium citrate | 1.4 (M) | |
| 7 | 1 | drop | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | sodium citrate | 1.4 (M) | |
| 9 | 1 | reservoir | HEPES | 100 (mM) |
