1PQ3

Human Arginase II: Crystal Structure and Physiological Role in Male and Female Sexual Arousal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 32
Unit cell lengths	142.994, 142.994, 127.328
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.227
Rwork	0.227
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Rmerge	0.098 *	0.280 *
Total number of observations	404391 *
Number of reflections	79653 *
Completeness [%]	99.3 *	98.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	4 *	Tris, Ammonium Sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	arginase	7 (mg/ml)
2	1	drop	BEC	5 (mM)
3	1	drop	Bicine	50 (mM)	pH8.5
4	1	drop	Tris-HCl	0.10 (M)	pH8.3-8.5
5	1	drop	ammonium sulfate	3.0 (M)
6	1	reservoir	Tris-HCl	0.10 (M)	pH8.3-8.5
7	1	reservoir	ammonium sulfate	3.0 (M)
8	1	reservoir	glycerol	20 (%(v/v))