1PMA
PROTEASOME FROM THERMOPLASMA ACIDOPHILUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 311.900, 209.700, 117.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.400 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 21.916 * |
| Data reduction software | MOSFLM (V. 5.23) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.096 |
| Number of reflections | 105440 |
| Completeness [%] | 83.0 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | proteasomes | 7 (mg/ml) | |
| 2 | 1 | 1 | MPS | 5 (mM) | |
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | PEG1000 | 10 (%) | |
| 6 | 1 | 1 | potassium phosphate | 0.1 (M) | |
| 7 | 1 | 2 | PEG1000 | 15 (%) | |
| 8 | 1 | 2 | potassium phosphate | 0.1 (M) |
