1PJ6

Crystal structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with folic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-05-01
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	C 2 2 2
Unit cell lengths	70.877, 222.506, 119.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.650
R-factor	0.15752
Rwork	0.156
R-free	0.19300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pj5
RMSD bond length	0.016
RMSD bond angle	1.634
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.700
High resolution limit [Å]	1.650	1.650
Rmerge	0.067	0.346 *
Number of reflections	106504
<I/σ(I)>	11.9
Completeness [%]	99.3	99
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	294	15% PEG 2000MME, 0.2 MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG5000 MME	15 (%)
3	1	reservoir		0.2 (M)	pH7.5
4	1	reservoir	HEPES	0.1 (M)