1PJ5

Crystal structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with acetate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	C 2 2 2
Unit cell lengths	71.381, 226.680, 120.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.600 *
R-factor	0.1619
Rwork	0.160
R-free	0.19800 *
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	1.578
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.096	0.521 *
Number of reflections	114767
<I/σ(I)>	7.4
Completeness [%]	97.2	96.8
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	15 % PEG 2000MME, 0.2 M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG5000 MME	15 (%)
3	1	reservoir		0.2 (M)	pH7.5
4	1	reservoir	HEPES	0.1 (M)