1P9P

The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2002-06-15
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	97.255, 97.255, 62.661
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.196
R-free	0.24700
Structure solution method	MAD
RMSD bond length	0.035
RMSD bond angle	2.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge		0.373
Number of reflections	12013
<I/σ(I)>		3.3
Completeness [%]	98.9	0.966
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	283	MPD, acetate buffer at pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K
1	VAPOR DIFFUSION, SITTING DROP	4.5	283	MPD, acetate buffer at pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K
1	VAPOR DIFFUSION, SITTING DROP	4.5	283	MPD, acetate buffer at pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K