1P4H
Crystal structure of glycogen phosphorylase b in complex with C-(1-acetamido-alpha-D-glucopyranosyl) formamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8015 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.980, 127.980, 115.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.060 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.20500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gfz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 59423 | |
| <I/σ(I)> | 17.1 | 2.9 |
| Completeness [%] | 99.4 | 92.8 |
| Redundancy | 6.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 289K |
