1P3Q
Mechanism of Ubiquitin Recognition by the CUE Domain of VPS9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-10-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979174, 0.979311, 0.95645 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.607, 45.887, 57.802 |
| Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.700 |
| Rwork | 0.260 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.367 |
| Number of reflections | 28955 | 2626 * |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 98.4 | 89.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 293 | PEG 3350, magnesium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris | 20 (mM) | pH7.7 |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.9-2.1 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2-8.8 |
