1P36

T4 LYOSZYME CORE REPACKING MUTANT I100V/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.843, 59.843, 95.579
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.000 - 1.450
R-factor	0.189
Rwork	0.187
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.015
RMSD bond angle	2.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.110	1.530
High resolution limit [Å]	1.450	1.450
Rmerge	0.060	0.238
Number of reflections	35041
<I/σ(I)>	8.1	3.1
Completeness [%]	97.9	86 *
Redundancy	7.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol