1P1Z

X-RAY CRYSTAL STRUCTURE OF THE LECTIN-LIKE NATURAL KILLER CELL RECEPTOR LY-49C BOUND TO ITS MHC CLASS I LIGAND H-2Kb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	149.047, 149.047, 64.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.260
Rwork	0.263
R-free	0.31560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	1.520 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.260	3.260
Rmerge	0.096	0.264 *
Number of reflections	12168 *	596 *
<I/σ(I)>		5.3
Completeness [%]	99.5	99.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	4 *	10% PEG 20000, 2% DIOXANE, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-10 (mg/ml)
2	1	reservoir	PEG20000	10 (%(w/v))
3	1	reservoir	dioxane	2 (%(v/v))
4	1	reservoir	bicine	0.1 (M)	pH9.0