1P15
Crystal structure of the D2 domain of RPTPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 31 |
| Unit cell lengths | 59.101, 59.101, 152.657 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.180 - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.100 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.041 * | 0.402 * |
| Total number of observations | 442014 * | |
| Number of reflections | 40834 | |
| Completeness [%] | 96.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | PEG 8000, sodium succinate, lithium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | sodium succinate | 0.1 (M) | pH5.5 |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | 0.25 (M) | ||
| 5 | 1 | drop | protein | 12 (mg/ml) |
