1P14
Crystal structure of a catalytic-loop mutant of the insulin receptor tyrosine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-30 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.472, 69.482, 88.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.900 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i44 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.298 |
| Total number of observations | 149169 * | |
| Number of reflections | 29657 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 170 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 20 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | 30 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 5 (mM) |
