1OZN
1.5A Crystal Structure of the Nogo Receptor Ligand Binding Domain Reveals a Convergent Recognition Scaffold Mediating Inhibition of Myelination
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0064, 1.0096, 0.9950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.206, 33.915, 60.221 |
| Unit cell angles | 85.02, 75.91, 67.96 |
Refinement procedure
| Resolution | 50.000 - 1.520 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.053 | 0.214 |
| Number of reflections | 33484 | |
| <I/σ(I)> | 34 | 7.4 |
| Completeness [%] | 96.6 | 96.4 |
| Redundancy | 14.4 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 298 | PEG4000, sodium chloride, sodium acetate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 35 (%) | |
| 3 | 1 | reservoir | 0.25 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH6.5 |
