1OYR

Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	99.790, 146.490, 152.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 3.100
Rwork	0.278
R-free	0.28890 *
Structure solution method	MIR
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	3.100	3.100 *
Rmerge	0.116 *	0.372 *
Number of reflections	40776	2021 *
Completeness [%]	99.1 *	99.8 *
Redundancy	5.2 *	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	37.5 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		20 (mM)