1OYP

Crystal Structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	51.310, 163.560, 166.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.750 *
Rwork	0.285
R-free	0.30600 *
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.750 *	2.750 *
Rmerge	0.097 *	0.332 *
Number of reflections	36688 *	1753 *
Completeness [%]	99.2 *	96.6 *
Redundancy	5.9 *	4.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	reservoir		1.0 (M)
3	1	reservoir		3.0 (M)