1OY7
Structure and Function Analysis of Peptide Antagonists of Melanoma Inhibitor of Apoptosis (ML-IAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 32 |
| Unit cell lengths | 83.878, 83.878, 94.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.1553 |
| Rwork | 0.152 |
| R-free | 0.21100 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1oxn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.137 * | 0.282 * |
| Number of reflections | 20139 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 99.0 * | 99.5 * |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 298 | sodium acetate, PEG 300, DTT , pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 10 (mM) | pH6.5 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.0 |
| 4 | 1 | reservoir | PEG300 | 5 (%(v/v)) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
