1OTX
Purine Nucleoside Phosphorylase M64V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2000-12-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.948 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 119.770, 119.770, 240.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 2.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecp |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.380 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.222 * |
| Number of reflections | 28781 | |
| <I/σ(I)> | 6.2 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.3 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | 50mM sodium citrate, 30%ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | pH8.0 | |
| 2 | 1 | drop | protein | 35 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 30 (%) | |
| 4 | 1 | reservoir | citrate | 50 (mM) | pH5.4 |
