1ORV
Crystal Structure of Porcine Dipeptidyl Peptidase IV (CD26)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05030 |
Spacegroup name | P 1 |
Unit cell lengths | 62.000, 118.180, 133.590 |
Unit cell angles | 112.76, 94.93, 91.14 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25200 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 25.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MAIN |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 1.800 * | |
Rmerge | 0.052 * | 0.352 * |
Total number of observations | 617050 * | |
Number of reflections | 322283 * | |
Completeness [%] | 96.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 2000, 0.1 M ammonium sulfate, 0.1 M Tris/HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 2000, 0.1 M ammonium sulfate, 0.1 M Tris/HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG2000 | 20-22 (%) | |
3 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |