1OOI

Crystal structure of LUSH from Drosophila melanogaster at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2002-05-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	55.370, 55.370, 66.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.040
R-factor	0.20083
Rwork	0.195
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oof
RMSD bond length	0.006
RMSD bond angle	1.172
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.110
High resolution limit [Å]	2.040	2.040
Rmerge	0.054	0.247
Total number of observations	112114 *
Number of reflections	7504
<I/σ(I)>	57.9	10.7
Completeness [%]	100.0	100
Redundancy	14.9	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	291	PEG 4000, MOPS, n-butanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	100 (mM)	pH8.0
2	1	drop	n-butanol	0.3 (%(v/v))
3	1	reservoir	MOPS	100 (mM)	pH6.5
4	1	reservoir	PEG4000	30 (%(w/v))
5	1	reservoir	n-butanol	0.3 (%(v/v))