1OK1

Decay accelerating factor (cd55) : the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	47.034, 55.133, 62.001
Unit cell angles	86.85, 84.92, 65.03

Refinement procedure

Resolution	16.000 - 2.600
R-free	0.25900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.006
RMSD bond angle	19.780 *
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	0.174
Number of reflections	16177
<I/σ(I)>	5.6	3.9
Completeness [%]	93.5	90
Redundancy	7.6	7.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 10% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))