1OJW
Decay accelerating factor (CD55): the structure of an intact human complement regulator.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 53.099, 51.892, 64.125 |
Unit cell angles | 78.39, 83.66, 63.22 |
Refinement procedure
Resolution | 25.000 * - 2.800* |
R-free | 0.28800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h03 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.600 * |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | TNT (5F) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 * | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.135 | 0.388 |
Number of reflections | 14573 | |
<I/σ(I)> | 15.37 | 3.43 |
Completeness [%] | 99.0 | 98.4 |
Redundancy | 6.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.6 | 4 * | 0.2 M AMMONIUM SULPHATE 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6 10% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
2 | 1 | reservoir | MPEG5000 | 20 (%(w/v)) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
4 | 1 | reservoir | glycerol | 10 (%(w/v)) |