1OBG

SAICAR-synthase complexed with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1997-02-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	69.770, 74.190, 76.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.800 - 2.050
R-factor	0.168
Rwork	0.166
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a48
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.820	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.070	0.190
Number of reflections	25206
<I/σ(I)>	17	3.6
Completeness [%]	98.5	98.5
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		40MM ASP. ASID, 10MM MGCL2, 25MM ATP, 2.26 M (NH4)2(SO4), 5, pH 4.50