1O9S
Crystal structure of a ternary complex of the human histone methyltransferase SET7/9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.690, 75.438, 69.099 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3i |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 54703 |
| <I/σ(I)> | 47.2 |
| Completeness [%] | 89.9 |
| Redundancy | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | pH 7.80 |
