1O9M
The Complex of a novel antibiotic with the Aminoacyl Site of the Bacterial Ribosome Revealed by X-Ray Crystallography.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.800, 46.550, 88.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.2507 |
| Rwork | 0.251 |
| R-free | 0.29810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j7t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 32243 | |
| <I/σ(I)> | 19.6 | 1.9 |
| Completeness [%] | 94.5 | 97.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 37 * | MPD, NACL, MGSO4, GLYCEROL, NA CACODYLATE, pH 6.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 40 (%) | |
| 2 | 1 | drop | antibiotic | 2 (mM) | |
| 3 | 1 | drop | 200 (mM) | pH6.4 | |
| 4 | 1 | drop | 300 (mM) |
