1O9M

The Complex of a novel antibiotic with the Aminoacyl Site of the Bacterial Ribosome Revealed by X-Ray Crystallography.

Summary for 1O9M

Entry DOI	10.2210/pdb1o9m/pdb
Descriptor	EUBACTERIAL A-SITE, 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranose, PAROMOMYCIN (RING 2), ... (6 entities in total)
Functional Keywords	antibiotic/rna, rna-antibiotic interactions, a-site, antibiotic-rna complex
Biological source	ESCHERICHIA COLI
Total number of polymer chains	2
Total formula weight	14671.23
Authors	Russell, R.,Murray, J.B. (deposition date: 2002-12-17, release date: 2003-03-27, Last modification date: 2023-12-13)
Primary citation	Russell, R.,Murray, J.B.,Lentzen, G.,Haddad, J.,Mobashery, S. The Complex of a Designer Antibiotic with a Model Aminoacyl Site of the 30S Ribosomal Subunit Revealed by X-Ray Crystallography J.Am.Chem.Soc., 125:3410-, 2003 Cited by PubMed Abstract: The ribosome is an important target for aminoglycoside antibiotics; however, the clinical effectiveness of aminoglycosides has diminished due to bacterial resistance mechanisms. Here we report the X-ray structure of a novel synthetic aminoglycoside bound to the A site of the ribosome, its target for manifestation of activity. The structure validates the in silico design paradigms for the antibiotic and reveals the molecular interactions made by this novel antibiotic in prokaryotes. PubMed: 12643685 DOI: 10.1021/JA029736H PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.4 Å)

Structure validation