1O9J

The X-ray crystal structure of eta-crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	80.869, 136.407, 84.988
Unit cell angles	90.00, 102.62, 90.00

Refinement procedure

Resolution	41.520 - 2.400
R-factor	0.184
Rwork	0.180
R-free	0.26200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bxs
RMSD bond length	0.011
RMSD bond angle	1.280 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.068	0.174
Total number of observations	159192 *
Number of reflections	67543	8412 *
<I/σ(I)>	12.2	7.2
Completeness [%]	96.5	94.7
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	25 MM BIS-TRIS-PROPANE PH 7.5, APPROXIMATELY 14% PEG 6,000.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Bis-Tris-HCl	25 (mM)
2	1	drop	EDTA	1 (mM)
3	1	drop	dithiothreitol	1 (mM)	pH6.5
4	1	drop	protein	8 (mg/ml)
5	1	reservoir	Bis-Tris propane	25 (mM)	pH7.5
6	1	reservoir	PEG6000	14 (%)