1O9J
The X-ray crystal structure of eta-crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.869, 136.407, 84.988 |
| Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
| Resolution | 41.520 - 2.400 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.26200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bxs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.174 |
| Total number of observations | 159192 * | |
| Number of reflections | 67543 | 8412 * |
| <I/σ(I)> | 12.2 | 7.2 |
| Completeness [%] | 96.5 | 94.7 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 25 MM BIS-TRIS-PROPANE PH 7.5, APPROXIMATELY 14% PEG 6,000. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Bis-Tris-HCl | 25 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | pH6.5 |
| 4 | 1 | drop | protein | 8 (mg/ml) | |
| 5 | 1 | reservoir | Bis-Tris propane | 25 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG6000 | 14 (%) |
