1O80

Crystal structure of IP-10 H-Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-25
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	84.560, 84.560, 116.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 * - 2.000
R-factor	0.264
Rwork	0.264
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rhp
RMSD bond length	0.009
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.078	0.520
Total number of observations	219378 *
Number of reflections	17139
<I/σ(I)>	20.1	3.3
Completeness [%]	98.4	99.8
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.75	16 *	10MG/ML PROTEIN, 0.1M TRIS-HCL BUFFER, PH 8.75, 3.3M SODIUM FORMATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.75
3	1	reservoir	sodium formate	3.3 (M)