1O5R

Crystal structure of adenosine deaminase complexed with a potent inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-30
Detector	RIGAKU RAXIS
Wavelength(s)	0.836
Spacegroup name	P 43 21 2
Unit cell lengths	78.440, 78.440, 137.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.350
R-factor	0.23
Rwork	0.228
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	AMoRE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.750	2.100
High resolution limit [Å]	2.060	2.060
Number of reflections	27278
Completeness [%]	99.0	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	Ammonium sulphate, PEG400, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K