Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-24 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.310, 74.830, 78.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.000 - 2.600 |
| Rwork | 0.218 |
| R-free | 0.29700 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.954 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.063 | 0.387 |
| Number of reflections | 24126 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 295 | 42.5% saturated ammonium sulphate, 100mM Tris ph 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.5 |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | sodium azide | 0.05 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 6 | 1 | reservoir | ammonium sulfate | 40-45 (%) | |
| 7 | 1 | reservoir | sodium azide | 0.1 (%) |
