1NY5

Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	94.765, 94.765, 195.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.600 * - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	22.600 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.080	0.415 *
Number of reflections	40276
<I/σ(I)>	35.3	3
Completeness [%]	99.4	83.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	sodium/potasium phosphate, citric acid,imidazole,methanol,glycerol,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		0.6-0.7 (M)
3	1	reservoir	citric acid	100 (mM)	pH6.5
4	1	reservoir	imidazole	10 (mM)
5	1	reservoir	methanol	3 (%(v/v))