1NVW
Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 184.046, 184.046, 178.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.000 * - 2.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25500 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1nvu |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.000 * | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.088 * | 0.463 * |
Total number of observations | 313273 * | |
Number of reflections | 40362 * | |
<I/σ(I)> | 20 | 4.6 |
Completeness [%] | 99.1 * | 96.7 * |
Redundancy | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-20 (mg/ml) | |
2 | 1 | reservoir | phosphate | 1.2-1.5 (M) | |
3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |