1NT3

HUMAN NEUROTROPHIN-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Collection date	1996-12
Spacegroup name	P 21 21 2
Unit cell lengths	37.434, 52.067, 64.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.219
Rwork	0.219
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NT3 PROTOMER OF 1BND
RMSD bond length	0.011
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.068	0.506
Number of reflections	6760
<I/σ(I)>	7.1	1.61
Completeness [%]	86.1	85.2
Redundancy	4.4	3.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	200 MM AMMONIUM SULFATE, 32% PEG 400, 100 MM MES AT PH 5.6, pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	reservoir	MES	100 (mM)