1NPM

NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-11
Spacegroup name	P 1
Unit cell lengths	38.400, 55.160, 65.370
Unit cell angles	95.38, 89.98, 110.46

Refinement procedure

Resolution	100.000 - 2.100
R-factor	0.186
Rwork	0.186
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4PTP
RMSD bond length	0.008 *
RMSD bond angle	1.535 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.240
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.198
Number of reflections	24944
<I/σ(I)>	8.5	3.1
Completeness [%]	90.7 *	74.5
Redundancy	2.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24.5 (mg/ml)
2	1	reservoir	HEPES	5 (mM)
3	1	reservoir		100 (mM)