1NOU

Native human lysosomal beta-hexosaminidase isoform B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	112.465, 112.465, 397.867
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 * - 2.400
R-factor	0.20266
Rwork	0.201
R-free	0.23100 *
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.590 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.090 *	0.314 *
Total number of observations	2772717 *
Number of reflections	58844	2894 *
Completeness [%]	99.5 *	99.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	Mahuran, D.J., (1980) Can. J. Biochem., 58, 287. *