1NNP

X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-ATPA at 1.9 A resolution. Crystallization without zinc ions.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-25
Detector	MARRESEARCH
Wavelength(s)	1.07600
Spacegroup name	P 21 21 2
Unit cell lengths	96.728, 121.469, 47.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.190
R-free	0.21700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GluR2:(S)-ATPA complex (zinc form).
RMSD bond length	0.006
RMSD bond angle	22.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.092	0.348
Number of reflections	40611
<I/σ(I)>	13.1	2.4
Completeness [%]	92.2	81.6
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	6 *	ammonium sulfate, PEG8000, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	GluR2-S1S2J	1.4 (mg/ml)
3	1	reservoir	ammonium sulfate	0.1 (M)
4	1	reservoir	sodium acetate	0.1 (M)	pH5.6
5	1	reservoir	PEG8000	18 (%)