1NKG

Rhamnogalacturonan lyase from Aspergillus aculeatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 43 21 2
Unit cell lengths	77.009, 77.009, 170.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 1.500
R-factor	0.164
Rwork	0.164
R-free	0.18600
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.108	0.292
Number of reflections	82997
<I/σ(I)>	16.71	4.5
Completeness [%]	100.0	100
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	277	20% PEG 4000, 9% PEG 400, 0.1M (NH4)2SO4, 0.1M sodium acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K