1NEK
Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0081 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 138.800, 138.800, 521.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 53727 | |
| Completeness [%] | 89.9 | 90 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | Tris-HCl, CaCl2, PEG 400, BaCl2, ethylene glycol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
