1NDD
STRUCTURE OF NEDD8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MACSCIENCE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.804, 64.972, 48.573 |
Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.600 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.067 | 0.452 |
Total number of observations | 232354 * | |
Number of reflections | 36579 | |
<I/σ(I)> | 20 | 1.9 |
Completeness [%] | 97.8 | 93.7 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.6 * | 21 * | pH 4.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris | 40 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | EDTA | 0.4 (mM) | |
5 | 1 | drop | dithiothreitol | 0.2 (mM) | |
6 | 1 | reservoir | citric acid | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2.2 (M) |