1NC1
Crystal structure of E. coli MTA/AdoHcy nucleosidase complexed with 5'-methylthiotubercidin (MTH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.82650 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.300, 69.300, 127.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.580 - 2.000 |
Rwork | 0.190 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nc3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.062 | |
Number of reflections | 30742 | |
Completeness [%] | 97.8 | 98.9 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 200, sodium acetate, sodium chloride, cobalt chloride, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |