1NAL
THE THREE-DIMENSIONAL STRUCTURE OF N-ACETYLNEURAMINATE LYASE FROM ESCHERICHIA COLI
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.800, 122.800, 198.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.010 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.200 * | 2.200 * |
| Rmerge | 0.096 * | |
| Total number of observations | 205465 * | |
| Number of reflections | 78145 * | |
| Completeness [%] | 55 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium phosphate | 75 (mM) | |
| 2 | 1 | reservoir | ammonium sulfate | saturated | |
| 3 | 1 | drop | sodium phosphate | 75 (mM) | |
| 4 | 1 | drop | ammonium sulfate | ||
| 5 | 1 | drop | protein | 17 (mg/ml) | |
| 6 | 1 | drop | sodium chloride | 0.1 (M) | |
| 7 | 1 | drop | sodium azide | 0.02 (%) | |
| 8 | 1 | drop | Tris-HCl | 25 (mM) |
