1N56
Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200HB |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-15 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.016, 102.358, 105.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jx4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.038 | 0.399 |
| Number of reflections | 39503 | |
| Completeness [%] | 93.5 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 0.1M Hepes, 100mM calcium acetate, 2.5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | calcium acetate | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | glycerol | ||
| 7 | 1 | 2 | calcium acetate | ||
| 8 | 1 | 2 | Hepes |
