1N48
Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200HB |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-20 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.546, 88.458, 92.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 2.200 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jx4 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.920 |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.049 | 0.239 |
Number of reflections | 29643 | |
Completeness [%] | 92.4 | 60.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 0.1 M Hepes, 100 mM calcium acetate, 2.5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | Hepes | ||
3 | 1 | 1 | calcium acetate | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 2 | PEG 3350 | ||
6 | 1 | 2 | glycerol | ||
7 | 1 | 2 | calcium acetate |