1N3S
Biosynthesis of pteridins. Reaction mechanism of GTP cyclohydrolase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.302, 124.302, 389.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.550 |
R-factor | 0.271 |
Rwork | 0.262 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.940 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.550 |
Number of reflections | 76546 |
Completeness [%] | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | Peg6000, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |