1N3L

Crystal structure of a human aminoacyl-tRNA synthetase cytokine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9800
Spacegroup name	P 21 21 2
Unit cell lengths	74.595, 162.392, 35.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.180
R-factor	0.1802
Rwork	0.180
R-free	0.21590 *
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	2.500 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.220
High resolution limit [Å]	1.180	1.180
Rmerge	0.054	0.699
Number of reflections	134129
<I/σ(I)>	55.2	1.004
Completeness [%]	93.0 *	59
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.38	279	(NH4)2SO4, NaH2PO4, K2HPO4, acetone, pH 6.38, VAPOR DIFFUSION, SITTING DROP, temperature 279K