1MWH

REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	70.891, 84.944, 249.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500
R-factor	0.243
Rwork	0.209
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	reovirus polymerase 1MUK
RMSD bond length	0.009
RMSD bond angle	1.719 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.590
High resolution limit [Å]	2.500 *	2.500
Rmerge	0.088 *	0.404 *
Number of reflections	49606
<I/σ(I)>	15	2
Completeness [%]	93.3 *	80.8 *
Redundancy	5-6 *	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	22 *	PEG4000, sodium chloride, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.7 (mg/ml)
2	1	drop	sodium HEPES	50 (mM)	pH7.8
3	1	drop	glycerol	10 (%)
4	1	drop		150 (mM)
5	1	drop	dithiothreitol	10 (mM)
6	1	drop		0.02 (%)
7	1	reservoir	PEG8000	7.5 (%)
8	1	reservoir	glycerol	10 (%)
9	1	reservoir	sodium HEPES	0.1 (M)	pH7.8