1MT5

CRYSTAL STRUCTURE OF FATTY ACID AMIDE HYDROLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	147.107, 272.019, 147.216
Unit cell angles	90.00, 115.21, 90.00

Refinement procedure

Resolution	50.000 * - 2.800
R-factor	0.22062
Rwork	0.218
R-free	0.23700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FAAH structure in an alternate spacegroup. Solved by MAD/MIRAS phasing.
RMSD bond length	0.029 *
RMSD bond angle	1.041 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.104 *	0.264 *
Number of reflections	173615
<I/σ(I)>	4.4
Completeness [%]	76.0 *	79 *
Redundancy	2.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG 6000, 2,4-methyl pentanediol, sodium citrate, lithium sulphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)	pH5.
3	1	reservoir	lithium sulfate	100 (mM)
4	1	reservoir	PEG6000	7 (%(w/v))
5	1	reservoir	MPD	5 (%(v/v))
6	1	reservoir	dithiothreitol	0-20 (mM)